Improvements in large-scale production of Tobacco Etch Virus protease.

Tobacco-etch-virus (TEV) protease is the workhorse of many laboratories in which protein expression is the linchpin of downstream experiments. TEV protease is remarkable in its sequence specificity as the cleavage sequence rarely appears in higher organisms and its ability to cleave fusion tag proteins from proteins of interest. Herein we report work done on large-scale production of TEV protease using different promotors, media, fusion tags, and expression platforms.

Prospects for high-throughput estimates of photosynthetic parameters in tropical trees using the Dynamic Assimilation™ Technique.

Amazônia is a species-rich region of immense importance to Earth's water and carbon cycling. Photosynthesis drives the global carbon cycle, so understanding photosynthetic differences across diverse landscapes is a key task of ecophysiology and ecosystem science. Unfortunately, due to physiological and logistical constraints, ground-based photosynthesis data in Amazônia remains scarce, and the 'traditional' steady-state method (SS) of gas exchange is slow and inefficient.

Single-Particle ICP-TOFMS Analysis of Aerosol Particulate Matter Released by Fused Deposition Modeling 3D Printers.

Additive manufacturing or 3D printing is rapidly growing in popularity. This growth brings increased interest in studying the byproducts released during 3D printing. In this work, we present a new method to collect and analyze the metal composition of individual particles released during the operation of fused deposition modeling (FDM) 3D printers using single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOFMS).