[Influence of Professional Contact with Plutonium-239 on Indicators of the Immune Status of the Personnel at Siberian Chemical Plant].

The results of the examination and monitoring of the personnel at the Siberian Chemical Plant (SChP) and adult population of Seversk are presented. The results of primary examination of the personnel who professionally contact the ionizing radiation (IR) from external sources and incorporated 239Pu showed that clinical symptoms of dysfunction of the immune system manifested themselves with a frequency of 75.30%.

Role of NF-kB, p53, and p21 in the regulation of TNF-α mediated apoptosis of lymphocytes.

The effect of recombinant TNF-α on programmed death of donor lymphocytes was studied in vitro. The proapoptotic effect of this cytokine is realized through transcription factors and cell cycle inhibitors. Incubation of lymphocytes with recombinant TNF-α revealed increased levels of NF-kB and p21 and reducted content of nonphosphorylated p53.

[The role of redox-dependent signal systems in the regulation of apoptosis under oxidative stress condition].

Programmed death of peripheral blood mononuclear cells from donors with acute inflammatory diseases (an acute appendicitis, a community-acquired pneumonia) was investigated under condition of oxidative stress in vitro and under effect of selective inhibitors of MAP-kinases JNK and p38. Levels of active and inactive forms of MAP-kinases, and factors of transcription were determined by immunoblotting (western blot analysis). The increase in the activity of apoptosis under condition of oxidative stress in vivo and during the acute inflammatory diseases is associated with the increase in the level of reactive oxygen species (ROS) in the cells.

Role of recombinant mitogen-activated protein kinases JNK and p38 in the regulation of apoptosis in blood mononuclear cells under conditions of oxidative stress in vitro.

Programmed death of peripheral blood mononuclear cells from healthy donors was studied during culturing with various concentrations of H(2)O(2) and selective inhibitors of JNK (SP600125) and p38 MAPK (ML3403). In vitro incubation of mononuclear leukocytes with 1 mM H(2)O(2) stimulated apoptotic cell death. Treatment with inhibitors (SP600125 and ML3403) during in vitro oxidative stress prevented the increase in the number of annexin-positive mononuclear cells.

Effect of recombinant interleukin-5, interleukin-3, and eotaxin on apoptosis in eosinophilic granulocytes.

We studied in vitro effects of recombinant interleukin-5, interleukin-3, and eotaxin on programmed death of eosinophils from healthy donors and patients with non-Hodgkin's lymphomas associated with severe blood eosinophilia. Interleukin-5 and eotaxin produced the most potent antiapoptotic effect on eosinophils from healthy donors. In patients with non-Hodgkin's lymphomas, spontaneous apoptosis in eosinophilic leukocytes was low and remained unchanged during incubation with recombinant proteins.