Galactose oxidase immobilized on silica in an analytical determination of galactose-containing carbohydrates.

Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst.

[Study of functional groups in the active center of alpha-galactosidase of Penicillium sp. 23].

Effect of metal ions and specific chemical reagents (EDTA, sodium aside, o-phenanthroline, n-chlormercurybenzoate, iodacetamide, N-ethylmaleinimide, L-cystein, dithiotreitol, beta-mercaptoethanol) on activity of alpha-galactosidase isolated from the culture liquid of micromycete Penicillium sp. 23 has been studied. It has been established that alpha-galactosidase is not metalloenzyme (lack of the inhibitor effects under treatment of enzyme by EDTA, sodium aside, o-phenanthroline).

[Functional groups in the alpha-galactosidase active site in Cladosporium cladosporioides].

The activity of alpha-galactosidase isolated from culture fluid of micromycete Cladosporium cladosporioides (Fres.) de Vries 16,038 has been studied as affected by cations, anions and specific chemical reagents (p-chlormercurybenzoate (p-ChMB), iodacetamide, N-ethylmaleimide, L-cysteine, dithiotreitol, beta-mercaptoethanol, EDTA, o-phenanthroline, sodium azide). It has been established that Ag+ ions inhibited competitively alpha-galactosidase at pH 4.

[The mitogenic properties of Fusarium graminearum and Rhodococcus erythropolis enzymes].

Blast transformation of peripheral blood lymphocytes in healthy people is studied by enzymes of the microbic origin possessing the lectin activity. Galactose oxidase of Fusarium graminearum IMV-F-1060 is shown to be mitogenically active with respect to the lymphocytes in the culture in vitro and may be one of home sources of lymphocytic mitogens for the laboratory investigations.

[The effect of potassium ferricyanide and copper (II) ions on the properties of immobilized galactose oxidase].

Preparations of galactosooxidase (EC 1.1.3.