Longitudinal and vertical ultrastructural lesion of crural muscle in post-thrombotic syndrome.

The vertical and longitudinal ultrastructural changes of the crural muscle (triceps surae) were studied in a selected patient material of 18 patients suffering from post-thrombotic syndrome. The transmission and scanning electron microscopic method helped in revealing that the damage had affected the contractile system, the mitochondria and the endoplasmic reticulum and in addition marked degenerative phenomena occurred. It was shown that vertically the impairments of the fine structure were localized in foci and involved the muscle layers to the same extent.

Ultrastructural damage of crural muscle in post-thrombotic syndrome.

The fine structure of crural muscles in 11 patients suffering from post-thrombotic syndrome was investigated. The contractile elements and the mitochondria were mainly involved in the pathological changes. Glycogen granules in increased number and strong lipid vacuolization were shown.

[Value of organ preservation with Collins-3 and Sacks II solutions of warm ischemic and re-perfused kidneys].

The protective effect of Collins or Sacks solutions was reduced after warm ischemia in preserved and reperfused dog kidneys. The degree of the developed pathologic alterations was connected with the length of the warm ischemic period. Functional changes as decrease of microcirculation and proteinuria and structural sequences as edema and diverse disintegration in glomeruli and tubuli were produced.

[Susceptibility of the myocardium to injury in decreased coronary circulation].

In the experiments intact dog hearts with ligated descendents, hearts with aorta insufficiency and others with a cardiopulmonary bypass were perfused. It was demonstrated that both ischaemia and relative oxygen deficiency induce within a comparatively short time the same type of irreversible damage in the structure of the subendocardial layers of the myocardium (lysis and rupture of the myofilament, swelling of the mitochondrium, rupture and lysis of the crista, clearing of the matrix). Consequently, the aimed protection of the myocardium during cardiac surgery is of the greatest importance.

Ultrastructural features of canine kidneys after preservation with hyperthermic solutions and subsequent reperfusion.

An analysis of the ultrastructural features of 35 canine kidneys after hypothermic preservation in Rheomacrodex, Collins3 and Sacks II solutions, and subsequent autologous reperfusion for 2 to 3 hours is presented. Signs of irreversibility (intramitochondrial crista fragmentation, cell membrane rupture, detachment of the brush borders in the area of the proximal tubular system) were confined to the Rheomacrodex-preserved kidneys. Other abnormal features (cytoplasmic vacuolation, mitochondrial swelling in the proximal tubules, vacuolation of the visceral epithelial cells, heteromorphism in the glomerular system) found in the acute stage of revascularization may be regarded as reversible and account for the transitory functional disorders of renal transplants.