AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering.

The inevitable transition from petrochemical production processes to renewable alternatives has sparked the emergence of biofoundries in recent years. Manual engineering of microbes will not be sufficient to meet the ever-increasing demand for novel producer strains. Here we describe the AutoBioTech platform, a fully automated laboratory system with 14 devices to perform operations for strain construction without human interaction.

Lamb dip CRDS of highly saturated transitions of water near 1.4 μm.

Doppler-free saturated-absorption Lamb dips were measured at sub-Pa pressures on rovibrational lines of HO near 7180 cm, using optical feedback frequency stabilized cavity ring-down spectroscopy. The saturation of the considered lines is so high that at the early stage of the ring down, the cavity loss rate remains unaffected by the absorption. By referencing the laser source to an optical frequency comb, transition frequencies are determined down to 100 Hz precision and kHz accuracy.

Direct, Precise Measurements of Isotopologue Abundance Ratios in CO Using Molecular Absorption Spectroscopy: Application to ΔO.

We present an ultrasensitive absorption spectrometer based on a 30 Hz/s stability, sub-kHz line width laser source coupled to a high-stability cavity-ring-down-spectroscopy setup. It provides direct and precise measurements of the isotopic ratios δO and δO in CO. We demonstrate the first optical absorption measurements of O anomalies in CO with a precision better than 10 ppm, matching the requirements for paleo-environmental applications.

Tools for managing and analyzing microarray data.

The microarray-based analysis of gene expression has become a workhorse for biomedical research. Managing the amount and diversity of data that such experiments produce is a task that must be supported by appropriate software tools, which led to the creation of literally hundreds of systems. In consequence, choosing the right tool for a given project is difficult even for the expert.