Publications by authors named "T Srinivasa Prasad"
The versatile nature of the urease enzyme makes it a valuable asset in biological and industrial contexts. The creation of bioconjugates using enzyme-polymer combinations has extended the shelf life and stability of urease. A triblock copolymer, PAM-co-PDPA-co-PMAA@urease (ADM@urease), was synthesized using acrylamide (AM), 2,5-dioxopyrrolidin-1-ylacrylate (DPA), methacrylic acid (MAA), and urease via the RAFT-Grafting-To polymerization method.
View Article and Find Full Text PDFBackground: Racial and ethnic disparities have been reported for HCC prognosis, although few studies fully account for clinically important factors and social determinants of health, including neighborhood socioeconomic status.
Methods: We conducted a retrospective multicenter cohort study of patients newly diagnosed with HCC from January 2010 through August 2018 at 4 large health systems in the United States. We used multivariable logistic regression and cause-specific Cox proportional hazard models to identify factors associated with early-stage HCC presentation and overall survival.
Article Synopsis
- The study addresses the difficulties in diagnosing pleural tuberculosis (TB) and introduces a new method called magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR) to improve detection.
- The method was tested on 99 clinical specimens and demonstrated a sensitivity of 85.2% and a specificity of 97.8%, which was significantly better than other existing diagnostic techniques.
- The researchers emphasize that while the results are promising, further testing with a larger sample size is needed to validate the effectiveness of the MB-AuNP-I-PCR method for diagnosing pleural TB.
Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium.
Cell Tissue Res
November 2024
The present study explores the advantages of enriching the freezing medium with membrane lipids and antioxidants in improving the outcome of prepubertal testicular tissue cryopreservation. For the study, testicular tissue from Swiss albino mice of prepubertal age group (2 weeks) was cryopreserved by slow freezing method either in control freezing medium (CFM; containing DMSO and FBS in DMEM/F12) or test freezing medium (TFM; containing soy lecithin, phosphatidylserine, phosphatidylethanolamine, cholesterol, vitamin C, sodium selenite, DMSO and FBS in DMEM/F12 medium) and stored in liquid nitrogen for at least one week. The tissues were thawed and enzymatically digested to assess viability, DNA damage, and oxidative stress in the testicular cells.
View Article and Find Full Text PDFIntroduction: Awareness, access, and use of clinical and direct-to-consumer (DTC) genetic tests has increased in recent years with documented disparities in these services. We provide updated data on test awareness and use, and report novel data on motivations and methods for accessing genetic tests.
Methods: Nationally representative data from the 2022 Health Information National Trends Survey (HINTS 6) were used to assess awareness and use of ancestry, personal trait, specific disease, and carrier testing by sociodemographic characteristics, examine reasons for undergoing tests, and methods of accessing them.