A mutation in the 5,10-methylenetetrahydrofolate reductase gene is not associated with preeclampsia in women of southeast Mexico.

Background: We investigated the possible association between C677T-5,10-methylenetetrahydrofolate (MTHFR) polymorphism and preeclampsia in women from the Yucatan Peninsula in Mexico.

Methods: We performed a comparative study among three groups of subjects: 148 preeclamptic women; 177 women with normal pregnancies, and 313 healthy volunteers as control group. All subjects were genotyped for C677T polymorphism in MTHFR gene by PCR amplification and digestion of the product with Hinf I restriction enzyme.

Polymorphism analysis of the internal transcribed spacer and small subunit of ribosomal RNA genes of Leishmania mexicana.

Leishmania mexicana causes a wide spectrum of clinical diseases. In spite of the variety of clinical forms, no data exist regarding genetic polymorphism of L. mexicana.

Seroepidemiological studies of cutaneous leishmaniasis in the Campeche state of México.

Seroepidemiological studies of cutaneous leishmaniasis were carried out in 169 individuals in a rural area of the Campeche state of México. Fifty showed cutaneous lesions suggestive of leishmaniasis, 70% were parasite positive and 96% skin test positive. An overall 40% positivity to skin test with Montenegro's antigen was found.