Manufacture and Evaluation of Light Emitting Diode Package Substrate Using Flexible Printed Circuit Board.

Unlike other light sources such as fluorescent lamps and incandescent bulbs, light-emitting diodes (LED) convert 70-80% of energy into heat. If the heat produced an LED chip is not effectively released, its luminous efficiency and lifespan are reduced. Therefore, as a method effectively release heat, an LED PKG substrate containing a heat-releasing material with excellent thermal conductance was fabricated, and its thermal resistance and luminous efficiency were analyzed.

Prevalence of porcine endogenous retroviruses in domestic, minature, and genetically modified pigs in Japan.

The present study examined the prevalence of porcine endogenous retroviruses (PERV) in pigs available in Japan using polymerase chain reaction (PCR) with primers specific for PERV-A, PERV-B, and PERV-C and for the full-length 5' to 3' long terminal repeat and using PCR-Southern blotting with env A-, env B-, env C-, and pol/pro-specific probes. All 376 pigs tested--Berkshire (B), Landrace (L), Duroc (D), Large White (W), miniature, and genetically modified triple-cross breed (LWD)--harbored both PERV-A and PERV-B genes. However, the prevalence of PERV-C differed among pigs: LWD, miniature, B, D, W, and L pigs were 100% (36/36), 83% (5/6), 68% (129/191), 52% (26/50), 21% (9/43), and 16% (8/50), respectively.

Production of alpha 1,3-galactosyltransferase gene-deficient pigs by somatic cell nuclear transfer: a novel selection method for gal alpha 1,3-Gal antigen-deficient cells.

The objective of the present study was to isolate alpha 1,3-galactosyltransferase (GalGT)-gene double knockout (DKO) cells using a novel simple method of cell selection method. To obtain GalGT-DKO cells, GalGT-gene single knockout (SKO) fetal fibroblast cells were cultured for three to nine passages and GalGT-null cells were separated using a biotin-labeled IB4 lectin attached to streptavidin-coated magnetic beads. After 15-17 days of additional cultivation, seven GalGT-DKO cell colonies were obtained from a total of 2.

Effects of recloning on the efficiency of production of alpha 1,3-galactosyltransferase knockout pigs.

Obtaining sufficient transgenic cells via selective cultivation of genetically manipulated somatic cells is difficult due to the limited number of cell divisions. Additionally, if irreversible mutations in a cell's chromosomes occur during selective cultivation and the cell is used as the nuclear donor, somatic cell nuclear transfer (SCNT) embryos often exhibit abnormal development. On the other hand, a SCNT method in which fetal cells derived from SCNT embryos are used as the nuclear donor (recloning method) is an effective technique for obtaining large quantities of transgenic cells.

Conceptual equivalence and health-related quality of life: an exploratory study in Japanese and Dutch cancer patients.

Research into the equivalence of Western and Japanese conceptualizations of health-related quality of life (HR-QOL) is scarce. We used the Western (European Organization for Research and Treatment of Cancer, EORTC-QLQ-C30) and the Japanese (HRQoL-20) questionnaire in order to analyze the conceptual similarity of HR-QOL factors, and the associations between specific symptom items with overall HR-QOL in Japanese (n=265) and Dutch (n=174) patients with various types of cancer. Both populations completed both instruments.