Publications by authors named "T Shemesh"
Proper distribution of organelles can play an important role in a moving cell's performance. During C. elegans gonad morphogenesis, the nucleus of the leading distal tip cell (DTC) is always found at the front, yet the significance of this localization is unknown.
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- The text introduces a protocol for using Neuronalyzer software to automatically reconstruct branched neuronal structures from noisy microscopy images.
- It outlines steps including loading images, denoising, segmenting, tracing, and extracting features like branch curvature and junction angles.
- The software is capable of batch processing and allows for statistical comparisons, making it versatile and effective in handling variations across different datasets.
Development of the C. elegans gonad has long been studied as a model of organogenesis driven by collective cell migration. A somatic cell named the distal tip cell (DTC) is thought to serve as the leader of following germ cells; yet, the mechanism for DTC propulsion and maneuvering remains elusive.
View Article and Find Full Text PDFThe presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures.
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- * This research combines microscopy and computational modeling to demonstrate that the dynamics of the endoplasmic reticulum (ER) significantly influence the distribution of MTs by creating contractile forces based on ER tubule junction density.
- * Disrupting the tethering and fusion of ER junctions leads to instability in the ER-MT system and the formation of MT bundles, highlighting the mechanical role of ER dynamics in organizing cellular structures, particularly in motile cells and neuronal axons.