Prikl Biokhim Mikrobiol
May 2001
Complexes of nucleic acids and acid nuclear proteins that are active toward human T-lymphocytes were isolated from cells of bakers' yeast Saccharomyces cerevisiae. The conditions of isolation of nucleoprotein complexes by acid extraction followed by microfiltration for concentration of macromolecular components were optimized. Gel filtration and electrophoresis were used to study the composition and molecular weights of components of the preparations obtained.
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November 1987
Immobilization of alpha-amylase from Bacillus subtilis by adsorption on carboxyl polyelectrolytes, copolymers of methacrylic acid and ethylene glycol dimethacrylate, was being investigated depending on the structure of the polymeric matrix. It is demonstrated that cation exchange resins can be successfully used for reversible sorption and immobilization of alpha-amylase. It was found that the more heterogeneous the structure of the polymeric carrier, the higher is reversibility of the enzyme adsorption.
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