Publications by authors named "T S Kalbfleisch"
The scientific community has long benefited from the opportunities provided by data reuse. Recognizing the need to identify the challenges and bottlenecks to reuse in the agricultural research community and propose solutions for them, the data reuse working group was started within the AgBioData consortium framework. Here, we identify the limitations of data standards, metadata deficiencies, data interoperability, data ownership, data availability, user skill level, resource availability, and equity issues, with a specific focus on agricultural genomics research.
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- A new reference genome assembly for the laboratory rat, named GRCr8, has been developed and is recognized by the Genome Reference Consortium.
- The assembly utilized advanced sequencing techniques (40× PacBio HiFi sequencing) and includes chromosome-level data, with 98.7% of sequences assigned to chromosomes, showing overall increases in chromosome sizes and notable expansions on Chromosomes 3, 11, 12, and Chr Y.
- The new assembly enhances genome quality by incorporating over 1100 new protein-coding genes and includes previously unannotated genes and centromeric sequences, supported by concurrent PacBio Iso-Seq data from multiple rat tissues.
Whole genome sequences (WGS) of 185 North American Thoroughbred horses were compared to quantify the number and frequency of variants, diversity of mitotypes, and autosomal runs of homozygosity (ROH). Of the samples, 82 horses were born between 1965 and 1986 (Group 1); the remaining 103, selected to maximize pedigree diversity, were born between 2000 and 2020 (Group 2). Over 14.
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- * New genetic studies have created a better version of the rat's DNA map, called GRCr8, which is more accurate and includes new information.
- * As technology improves, researchers are now able to make more detailed DNA maps of different rat breeds, helping to unlock even more secrets about their genetics.
Pinholin S68 is a viral integral membrane protein whose function is to form nanoscopic "pinholes" in bacterial cell membranes to induce cell lysis as part of the viral replication cycle. Pinholin can transition from an inactive to an active conformation by exposing a transmembrane domain (TMD1) to the extracellular fluid. Upon activation, several copies of the protein assemble via interactions among a second transmembrane domain (TMD2) to form a single pore, thus hastening cell lysis and viral escape.
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