Publications by authors named "T S Harter"
Article Synopsis
- Marine fish, like splitnose rockfish, release extra hydrogen (H) through special proteins in their gills when in stressful environments with too much carbon dioxide (hypercapnia).
- Even though their blood shows stable pH levels, the fish don't increase the amount of proteins needed for this process but instead change how those proteins are arranged and used in their gills.
- This ability to adapt without needing new proteins helps fish handle changes in their environment, especially with climate change affecting ocean conditions.
Aim: To identify the physiological role of the acid-base sensing enzyme, soluble adenylyl cyclase (sAC), in red blood cells (RBC) of the model teleost fish, rainbow trout.
Methods: We used: (i) super-resolution microscopy to determine the subcellular location of sAC protein; (ii) live-cell imaging of RBC intracellular pH (pH) with specific sAC inhibition (KH7 or LRE1) to determine its role in cellular acid-base regulation; (iii) spectrophotometric measurements of haemoglobin-oxygen (Hb-O) binding in steady-state conditions; and (iv) during simulated arterial-venous transit, to determine the role of sAC in systemic O transport.
Results: Distinct pools of sAC protein were detected in the RBC cytoplasm, at the plasma membrane and within the nucleus.
In groundwater modeling studies, accurate spatial and intensity identification of water sources and sinks is of critical importance. Precise construction data about wells (water sinks) are particularly difficult to obtain. The collection of well log data is expensive and laborious, and government records of historic well log data are often imprecise and incomplete with respect to the precise location or pumping rate.
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