[Li/Na exchange and Li active transport in human lymphoid cells U937 cultured in lithium media].

Lithium transport across the cell membrane is interesting in the light of general cell physiology and becau- se of its alteration during numerous human diseases. The mechanism of Li4 transfer has been studied mainly in erythrocytes with a slow kinetics of ion exchange and therefore under the unbalanced ion distribution. Prolife- rating cultured cells with a rapid ion exchange have not been used practically in study of Li4 transport.

[Changes in K+, Na+ and Cl- contents and K+ and Cl- fluxes during apoptosis of U937 cells by staurosporine. On the mechanism of cell dehydration in apoptosis].

K+, Na+ and Cl- balance and K+ (Rb+) and 36Cl fluxes in during apoptosis of U937 cells caused by 0.2 or 1 microM staurosporine were studied by flame emission and radiotracer techniques. It is found that monovalent ion redistribution accounts for 2/3 of all decrease in the amount of intracellular osmolytes in apoptotic cells while 1/3 is due to the loss of other intracellular osmolytes.

[The characters and specific features of new human embryonic stem cells lines].

Four continuous human embryonic stem cell lines (SC1, SC2, SC3 and SC4), derived from the blastocysts has been described. The cell lines were cultivated on mitotically inactivated human feeder cells. The cell lines SC1 and SC2 have passed through 150 population doublings and the cell lines SC3 and SC4 -- near 120 populations doublings, which exceeds Hayflick limit sufficiently.

[The influence of immobilized laminin on karyotypic variability in two karyotypic different variants of the Indian muntjac skin fibroblast cell line].

The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblasts "markerless" cell line MT and in its karyotypic variant MT(d) cultivated on the laminin 2/4 coated surface. In cell line MT preincubated in serum free medium for 2.5 and 1 h and then cultivated on the laminin-coated surface in the serum-containing medium for 1, 2 and 3 days, the character of cell distribution for the chromosome number has changed.

[The influence of immobilized fibronectin on karyotypic variability of two rat kangaroo kidney cell lines].

The numerical and structural karyotypic variability has been investigated in "markerless" Rat kangaroo kidney cell lines NBL-3-17 and NBL-3-11 when cultivating on a fibronectin-coated surface. In cell line NBL-3-17, cultivated on the fibronectin-coated surface for 1, 2, 4 and 8 days, the character of cell distribution for the chromosome number has changed. These changes involve a significant decrease in frequency of cells with modal number of chromosomes, and an increase in frequency of cells with lower chromosomal number.