Improving Interpretation Consistency of Serum Capillary Electrophoresis by Development of Quantitative Graphic Indexes.

Capillary zone electrophoresis-immunosubtraction (CZE-IS) is an essential laboratory test in diagnosing plasma cell neoplasms. However, the current interpretation of the test results is subjective. To evaluate CZE-IS in a more precise manner, this study proposed five key indexes, namely sharpness index, light chain index, immunoglobulin G index, immunoglobulin A index, and immunoglobulin M index.

Retrospective Analysis to Optimize the Detection of Exon 14 Skipping Mutations in Non-Small Cell Lung Cancer.

Our study optimized ex14 skipping mutation detection by analyzing 223 Oncomine Focus Assay-positive cases using Pan Lung Cancer PCR Panel and reverse transcription (RT)-PCR. Among the 11 ex14 skipping mutation-positive cases (average read counts: 1390), 2 with Oncomine Focus Assay read counts of 2540 and 10,177 were positive on all platforms. Those with Oncomine Focus Assay read counts ranging from 179 to 612 tested negative elsewhere.

Objective indexes for comparing platelet usage among peer hospitals during the COVID-19 pandemic: A cross-sectional study.

Background And Aims: Besides hospital size, clinical diagnosis and severity of patient cases determine the total platelet usage. Therefore, the appropriateness of platelet usage could not be compared simply with the total units of platelet usage in each hospital. This study aimed to objectively monitor and analyze platelet usage after implementing a single-unit issuing policy for each platelet transfusion in our hospital in October 2020.

Evaluation of Platelet Alloimmunization by Filtration Enzyme-Linked Immunosorbent Assay.

The current methods for detecting antiplatelet antibodies are mostly manual and labor-intensive. A convenient and rapid detection method is required for effectively detecting alloimmunization during platelet transfusion. In our study, to detect antiplatelet antibodies, positive and negative sera of random-donor antiplatelet antibodies were collected after completing a routine solid-phase red cell adherence test (SPRCA).

Preservation of red blood cell antigenicity in a new storage solution .

Introduction: Red blood cell (RBC) storage solution is used for suspending and preserving RBCs for later use in immunohematology testing. Proper RBC preservation is crucial for obtaining accurate results in RBC phenotyping and pretransfusion antibody screening tests. Haemolysis or RBC antigen degradation during storage can result in inaccurate RBC phenotyping, thereby decreasing the sensitivity of pretransfusion antibody screening and identification assays.