Publications by authors named "T R L Klei"
Hemostatic conditions following autologous transfusion of fresh vs stored platelets in experimental endotoxemia: an open-label randomized controlled trial with healthy volunteers.
Res Pract Thromb Haemost
November 2024
Article Synopsis
- The study aimed to investigate how fresh and stored platelet concentrates (PCs) behave during inflammation, specifically in a controlled human model of endotoxemia induced by lipopolysaccharide (LPS).
- Researchers found that LPS increased markers related to coagulation and clotting, leading to a generally lower clotting ability in subjects compared to controls, while platelet transfusions improved clotting time and strength in those with endotoxemia.
- Despite these improvements in coagulation, there were no significant differences noted between the effects of fresh versus stored platelets on coagulation parameters like thrombin-antithrombin complex (TATc) or extracellular vesicles (EVs).
Background And Objectives: Plasma components are visually inspected, and non-transparent, turbid units are rejected for transfusion and fractionation. Additionally, in case a plasma component is deemed lipaemic, there is conflicting data on the accompanying red cell concentrate (RCC) in vitro quality. As visual inspection of plasma turbidity is a subjective method, we aimed to devise an objective measurement using a quick, non-invasive, table-top spectrophotometry-based method.
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- - Thrombocytopenia in preterm newborns can lead to increased bleeding risk, and recent findings suggest that using adult-derived platelet transfusions may be harmful due to developmental mismatches; hence there's interest in using cord blood-derived platelet concentrates (CBPCs) as a safer alternative.
- - The study conducted various methods to produce CBPCs from cord blood collected after near-term and full-term pregnancies and compared these to adult-derived platelet concentrates (PCs) in terms of quality parameters during storage.
- - Results showed that while CBPCs were generally comparable to control PCs, they exhibited lower pH and platelet concentration along with elevated apoptosis markers, indicating a need for improved processing and storage practices for low-volume platelets.
Background And Objectives: Near-infrared (NIR) light has been successfully applied to improve the quality of mouse platelets during storage. Because it is suspected that the mitochondria contain the primary photon acceptor, we hypothesized that human platelets for transfusion may be affected similarly and could benefit from NIR light treatment.
Materials And Methods: The optimal light dose was determined using portions of platelet concentrates (PCs) in PAS-E.
Thrombocytopenia (defined as a platelet count <150×10/L) is a common condition in preterm neonates and may occur in 18-35% of all infants admitted to the Neonatal Intensive Care Unit (NICU). Neonatal platelet functionality in terms of reactivity is often described as reduced compared to adults, even in healthy, term neonates. However, this platelet "hyporeactivity" does not correspond to a global functional impairment of the normal delicately balanced neonatal hemostatic system.
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