Chemical- and photo-activation of protein-protein thiol-ene coupling for protein profiling.

The thiol-ene reaction between an alkene and a thiol can be exploited for selective labelling of cysteine residues in protein profiling applications. Here, we explore thiol-ene activation in systems from chemical models to complex cellular milieus, using UV, visible wavelength and redox initiators. Initial studies in chemical models required an oxygen-free environment for efficient coupling and showed very poor activation when using a redox initiator.

Resilience Profiles among Grandparents Raising Grandchildren in the United States: White and Black Differences.

Background And Objectives: Grandparents raising grandchildren face many challenges and stress regardless of race and ethnicity; however, they are generally resilient. The present study aims to classify resilience profiles of these grandfamilies using a person-centered approach and examine the association of race and ethnicity with these profiles.

Research Design And Methods: The present study analyzed cross-sectional survey data collected from grandparents raising grandchildren in the United States (N = 287).

Differences in Postoperative Disposition by Kidney Disease Severity: A Population-Based Cohort Study.

Rationale & Objective: People with advanced kidney disease undergo more non-cardiac operations compared to the general population, with a higher risk of perioperative cardiac events and death. However, little is known about the associations between severity of preoperative kidney dysfunction with postoperative length of hospitalization and discharge disposition; these were the focus of this study.

Study Design: Population-based retrospective cohort.

Amino acid profiles for red wolves (Canis rufus) managed under human care are significantly different compared to the profiles of domestic dogs (Canis familiaris).

Objective: To establish a reference interval for amino acid profiles for healthy red wolves (Canis rufus).

Methods: Heparinized plasma of 48 red wolves was collected between August 2023 and April 2024 and sent to the University of California-Davis Amino Acid Laboratory for analysis. Reference intervals were created using the published American Society for Veterinary Clinical Pathology reference interval guidelines.

Cognitive aging outcomes are related to both tau pathology and maintenance of cingulate cortex structure.

Introduction: Successful cognitive aging is related to both maintaining brain structure and avoiding Alzheimer's disease (AD) pathology, but how these factors interplay is unclear.

Methods: A total of 109 cognitively normal older adults (70+ years old) underwent amyloid beta (Aβ) and tau positron emission tomography (PET) imaging, structural magnetic resonance imaging (MRI), and cognitive testing. Cognitive aging was quantified using the cognitive age gap (CAG), subtracting chronological age from predicted cognitive age.