Single-molecule microscopy reveals that importin α slides along DNA while transporting cargo molecules.

Importin α is a crucial player in the nucleocytoplasmic transport of nuclear localization signal (NLS)-containing cargo proteins and is suggested to bind to DNA directly. We hypothesized that importin α, after binding to DNA, may move along DNA via sliding or hopping. We investigated the movement dynamics of importin αs fused to AcGFP along DNA using single-molecule fluorescence microscopy and single-tethered DNA arrays.

Temporal and spatial pattern analysis of escaped prescribed fires in California from 1991 to 2020.

Background: Prescribed fires play a critical role in reducing the intensity and severity of future wildfires by systematically and widely consuming accumulated vegetation fuel. While the current probability of prescribed fire escape in the United States stands very low, their consequential impact, particularly the large wildfires they cause, raises substantial concerns. The most direct way of understanding this trade-off between wildfire risk reduction and prescribed fire escapes is to explore patterns in the historical prescribed fire records.

Comparative study of phenotypic and genotypic expression of virulence factors in colonizing and pathogenic carbapenem resistant Acinetobacter baumannii (CRAB).

Carbapenem resistant Acinetobacter baumannii has evolved as the most troublesome microorganism with multiple virulence factors. Biofilm formation, porins, micronutrient capturing mechanism and quorum sensing, provide protection against desiccation, host-pathogen killing and enhance its persistence. The conservation of these factors between colonizing and pathogenic carbapenem resistant A.

Emergent symmetries in prethermal phases of periodically driven quantum systems.

Periodically driven closed quantum systems are expected to eventually heat up to infinite temperature ; reaching a steady state described by a circular orthogonal ensemble (COE). However, such finite driven systems may exhibit sufficiently long prethermal regimes; their properties in these regimes are qualitatively different from that of their corresponding infinite temperature steady states. These, often experimentally relevant, prethermal regimes host a wide range of phenomena; they may exhibit dynamical localization and freezing, host Floquet scars, display signatures of Hilbert space fragmentation, and exhibit time crystalline phases.

Monotherapy or combination therapy of Oleanolic acid? From therapeutic significance and drug delivery to clinical studies: A comprehensive review.

Oleanolic acid (OA) is a pentacyclic triterpenoid molecule widely distributed throughout medicinal plants. This naturally occurring OA has attracted considerable interest due to its wide range of pharmacological characteristics, notably its cytotoxic effects on various human cancer cell lines, making it a potential candidate for extensive therapeutic uses. In vivo studies have shown that OA possesses hepatoprotective, cardioprotective, anti-inflammatory and anti-microbial properties.