Development and evaluation of ursolic acid-loaded poly(lactic--glycolic acid) nanoparticles in cholangiocarcinoma.

Cholangiocarcinoma (CCA), an epithelial biliary tract malignancy, is a significant health concern in the Greater Mekong Subregion, particularly in northeastern Thailand. Prior to the development of advanced stages, CCA is typically asymptomatic, thereby limiting treatment options and chemotherapeutic effectiveness. Ursolic acid (UA), a triterpenoid derived from plants, was previously discovered to inhibit CCA cell growth through induction of apoptosis.

Multifunctional fluorescent Eu-MOF probe for tetracycline antibiotics and dihydrogen phosphate sensing and visualizing latent fingerprints.

The contamination of tetracycline antibiotics and dihydrogen phosphate (HPO ) in food and the environment is one of the major concerns for human health. Herein, a water-stable carboxyl-functionalized europium metal-organic framework (Eu-MOF) was prepared and demonstrated, for the first time, as a dual-responsive fluorescent sensor of tetracycline antibiotics (oxytetracycline (OTC), tetracycline (TC), and doxycycline (DOX)) and HPO fluorescent turn-on and turn-off, respectively. Eu-MOF presents a sensitive and selective detection of OTC with a rapid response time (1 min) and good anti-interference ability.

Rapid detection of cancer DNA in human blood using cysteamine-capped AuNPs and a machine learning-enabled smartphone.

DNA methylation occurs when a methyl group is added to a cytosine (C) residue's fifth carbon atom, forming 5-methylcytosine (5-mC). Cancer genomes have a distinct methylation landscape (Methylscape), which could be used as a universal cancer biomarker. This study developed a simple, low-cost, and straightforward Methylscape sensing platform using cysteamine-decorated gold nanoparticles (Cyst/AuNPs), in which the sensing principle is based on methylation-dependent DNA solvation.

Insight into the Covalently Oriented Immobilization of Antibodies on Gold Nanoparticle Probes to Improve Sensitivity in the Colorimetric Detection of .

In this work, the covalently oriented conjugation of monoclonal antibody (mAb-) to amino-terminated oligo(ethylene glycol)-capped gold nanoparticles (NH-TEG-AuNPs) was studied. After NH-TEG-AuNPs were synthesized, the amino-terminated ligands on the particles were then linked to the carboxyl groups in the mAb- through EDC/NHS chemistry. By maintaining the pH of the solution at ∼5, the Fc region of the antibody could preferably attach to the particle surface, providing a specific Fab region that was available for binding with the target pathogen.