Reduced arterial elasticity due to surgical skeletonization is ameliorated by abluminal PEG hydrogel.

Arteries for bypass grafting are harvested either with neighboring tissue attached or as skeletonized vessels that are free of surrounding tissue. There are significant benefits to skeletonization, but reports suggest that skeletonized vessels may develop structural defects and are at risk for atherosclerosis. We investigated the specific short-term effects of skeletonization on carotid artery biomechanics and microanatomy in a rabbit model.

Temperature dependence of Ti:Sapphire fluorescence spectra for the design of cryogenic cooled Ti:Sapphire CPA laser.

The fluorescence spectra of titanium doped sapphire (Ti:Sapphire) crystals were measured for temperature ranging from 300K to 77K. The resulting gain cross-section line shapes were calculated and used in a three-dimensional amplification model to illustrate the importance of the precise knowledge of these fluorescence spectra for the design of cryogenic cooled Ti:Sapphire based chirped-pulse laser amplifiers.

Quantitative semi-automated analysis of morphogenesis with single-cell resolution in complex embryos.

A quantitative understanding of tissue morphogenesis requires description of the movements of individual cells in space and over time. In transparent embryos, such as C. elegans, fluorescently labeled nuclei can be imaged in three-dimensional time-lapse (4D) movies and automatically tracked through early cleavage divisions up to ~350 nuclei.

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination.

A key challenge when imaging living cells is how to noninvasively extract the most spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane-illumination microscopy limits excitation to the information-rich vicinity of the focal plane, providing effective optical sectioning and high speed while minimizing out-of-focus background and premature photobleaching. Here we used scanned Bessel beams in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets (<0.

Spatiotemporal dynamics of cross-polarized wave generation.

We use time-domain spatially and spectrally resolved interferometry to investigate cross-polarized wave (XPW) generation in barium fluoride. We find that the XPW pulse is square root of 3 smaller than the input in the spatiotemporal domain, regardless of input chirp. Additionally, we calculate a temporally dependent focal length resulting from the nonlinear interaction and discuss its implications.