[Influence of temperature on the calibration curves in IRMA for neuron-specific enolase and its physicochemical interpretation].

Background: immunoradiometric assay (IRMA) is one of the principal methods used for the analytical determination of neuron specific enolase (NSE) concentration. We studied the influence of temperature on the calibration curves obtained by this method, and a physicochemical justification based on two theoretical models is proposed.

Material And Methods: we used a commercially available RIA kit for NSE and a gamma counter.