The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes.

The objective of present study was to determine the effect of T-2 toxin and deoxynivalenol (DON) on DNA fragmentation in spleen leukocytes and oxidative stress in chickens, and furthermore, to evaluate the potential of dietary nucleotides in reduction of toxin-induced DNA damage. Male broiler chickens were exposed to 10mg/kg feed of either T-2 toxin or DON with or without addition of dietary nucleotides. After 17 days of treatment DNA damage of spleen leukocytes was measured by Comet assay, lipid peroxidation was studied by malondialdehyde (MDA), total antioxidant status (TAS) of plasma and glutathione peroxidase (GPx) assays, and the hepatotoxicity was studied by measuring plasma liver enzyme levels (ALT, AST and GGT) levels.

Efficiency of apples, strawberries, and tomatoes for reduction of oxidative stress in pigs as a model for humans.

Objective: The present study investigated the potency of apples, strawberries, and tomatoes in decreasing oxidative stress in pigs as a model for humans.

Methods: Forty-eight growing pigs were individually penned in metabolic cages and divided into six groups. All groups received isocaloric daily rations composed of a basal diet supplemented with starch, linseed oil, linseed oil and apples, linseed oil and strawberries, linseed oil and tomatoes, or linseed oil, apples, strawberries and tomatoes.

Relevance of meat fat content and fruit and vegetable intake for the oxidative status of pigs.

Background: The aim of the present study was to evaluate the effect of substituting lean meat with fat meat on oxidative stress in a diet with or without fruit and vegetables.

Methods: Thirty-two pigs were divided into groups and fed isocaloric daily rations: LM+FV (balanced diet with lean meat and fruit and vegetables); FM+FV (as LM+FV, but lean meat was substituted with fat meat); LM-FV (as LM+FV, but without fruit and vegetables), and FM-FV (as FM+FV, but without fruit and vegetables). Oxidative stress was evaluated by measuring the 24-hour urine malondialdehyde excretion rate, the degree of leukocyte nuclear DNA damage, the concentration of tocopherols in blood plasma, erythrocyte glutathione peroxidase activity and the total antioxidant status of plasma.

Wheat bran and oat bran effectively reduce oxidative stress induced by high-fat diets in pigs.

Aim: To evaluate the influence of wheat bran and oat bran on the oxidative stress induced by a high proportion of fat in the diet.

Methods: Forty-eight growing pigs were penned individually and after an adaptation period divided into four groups. All groups received isocaloric daily rations composed of basal diet which was then supplemented with: starch (controls; CONT), linseed oil (OIL), linseed oil and wheat bran, or linseed oil and oat bran.