[Penicillium chrysogenum mycelial productivity in the 1st phase of penicillin biosynthesis].

The course of the mycelium low productivity during the first phase of the usual two-stage process of penicillin biosynthesis was studied. It was found that the low productivity of the mycelium at the beginning of the fermentation process was probably associated with catabolic regression of the penicillin-producing system. The high specific growth rate registered in the experiments (0.

[Metabolites toxic for penicillin biosynthesis in P. chrysogenum fermentation].

Substances toxic for biosynthesis of penicillin accumulated in the medium at the end of the process during penicillin fermentation. Accumulation of such substances was associated with the mycelium autolysis. Addition of nutrient substances as soon as they are consumed prevented autolysis of the mycelium and accumulation of the toxic metabolites.

[Biosynthesis of penicillin and the biphasal development of a P. chrysogenum culture].

The two-phase pattern of penicillin biosynthesis was observed only under definite cultivation conditions. When the conditions of the culture growth changed, the productivity curve also changed. The most high productivity levels on the glucose medium and the medium with glucose and lactose were noted at the beginning and in the middle of the process respectively.

[Carbon metabolism under conditions of regulated penicillin biosynthesis].

Carbon metabolism of P. chrysogenum under conditions of periodical addition of the nutrients was studied. It was found that a proper rate of the carbon source addition to the culture was of significant importance for intensive biosynthesis.