[Effect of citric acid on synthesis of surfactants in Rhodococcus erythropolis IMV Ac-5017].

Expediency of sodium citrate (regulator of lipids synthesis) substitution is shown in the medium of cultivation of Rhodococcus erythropolis IMV Ac-5017 with ethanol (or hexadecane) and fumarate (gluconeogenesis precursor) by citric acid for pH maintenance at the level optimal for synthesis of surfactants. It has been established the maximum synthesis of surfactants of R. erythropolis IMV Ac-5017 was observed at pH 8.

[Peculiarities of ethanol oxidation by the producer of surface-active substances Acinetobacter calcoaceticus K-4].

Activity of key-enzymes of C2-metabolism was determined in the cells of strain-producer of surface-active substances Acinetobacter calcoaceticus K-4 grown on ethanol. It is shown that ethanol and acetaldehyde oxidation in the strain K-4 is performed by pyroquinolinquinon (PQQ) and 4-nitroso-N,N-dimethylaniline (NDMA)-dependent dehydrogenases. Activity of NDMA-dependent enzymes was maximum (100-300 nmol min(-1) mg(-1) of protein) in the early exponential growth phase of A.

[The influence of Acinetobacter calcoaceticus K-4 surface-active substances on the efficiency of microbial destruction of oil pollutants].

The possibility of the use of Acinetobacter calcoaceticus K-4 surface-active substances (SAS) for water purification from oil was shown. The efficiency of oil degradation (2.6 g/l) in the presence of SAS preparations (5-15 %) in the form of postfermentation of cultural liquid or its supernatant was established to be 81-95 %.

[Peculiarities of surface-active trehalose mycolates synthesis of Rhodococcus erythropolis EK-1].

It has been established that activity of trehalosephosphate synthase (key enzyme of trehalosemycolates synthesis) under cultivation of Rhodococcus erythropolis EK-1 on the medium with n-hexadecane was 10 times higher, and amount of synthesized surface-active substances 4 times higher as compared to the growing of EK-1 strain on ethanol. Introduction of 0.2% of fumarate and 0.

[Biodegradation of surface-active substances of Acinetobacter calcoaceticus K-4].

A capacity of microorganisms of different taxonomic groups to assimilate surface-active substances (surfactants) of Acinetobacter calcoaceticus K-4 as a single source of carbon and energy has been established. It was shown that A. calcoaceticus K-4 cannot use its own surfactants as a source of carbon nutrition.