[Transfer of the functional genes into eucaryotic cells by neutral phospholipid liposomes].

Transfection of plasmid DNAs containing b-galactosidase gene (pQE-LacZ) or alkaline phosphatase (pCSEAP) into L929 cell line using was studied. The complexes between plasmid DNA and liposomes containing Ca ions and glycyrrhizic acid or &-tocopherol caused successful transfection of functional genes into L929 cells. The efficiency of transfection of plasmid DNAs into L929 cells using polynucleotide-metallo(II)-liposome complexes were 30-50% from the efficiency value of calcium phosphate coprecipitation transfection.

Rat liver neutral alpha-glucosidase: isolation and characterization.

Neutral alpha-glucosidase was isolated from rat liver by Sephadex G-150 gel filtration and polyacrylamide gel electrophoresis at pH 8.9. The enzyme was found to exist in two major forms: alpha-glucosidase AB and alpha-glucosidase C.

[Effect of biogenic amines on glycogen degradation in isolated rat hepatocytes].

Serotonin and tyramine affected dissimilarly the enzyme activity involved in degradation of glycogen in isolated rat hepatocytes. Serotonin inhibited hydrolytic enzymes acid alpha-glucosidase, alpha-amylase and amylo-1,6-glucosidase but activated phosphorylase inducing a decrease of glycogen content in cells. Tyramine inhibited acid alpha-glucosidase and amylo-1,6-glucosidase, did not affect the alpha-amylase and phosphorylase activities and increased content of glycogen in cells.