[Clostridium difficile and diarrhea in infants in the first half-year of life].

In order to study a possible etiological relationship between Clostridium and diarrhea in children of the first half year of life and to characterize the colonization of the intestine with these bacteria, bacteriological investigations of feces were carried out in neonates and babies aged 1, 4 and 14 days and 1, 3 and 6 months. The development of the children and their health status were monitored under home conditions. It has been established that the colonization of the neonates' intestine with Clostridium including C.

[A study of the features of E. coli bacteroid infection in experiments on mice].

Experiments on the intraperitoneal infection of F1 (CBA X C57BL/6) mice with bacteroids of the group Fragilis in combination with E. coli have revealed the presence of a synergic effect leading to more pronounced (greater frequency of abscess formation) and even qualitatively new (necrotic lesions of the liver) pathological changes in comparison with those observed in monoinfections. Bacteroides fragilis strain 1/1087 gamma has been shown to have greater virulence than that of Bacteroides vulgaris strain 18; their virulence was determined from the capacity of microorganisms for survival in the abdominal cavity of mice, as well as from their capacity for inducing the formation of necrotic lesions and abscesses.

[Rapid methods in culturing nonsporulating anaerobes and determining their antibiotic sensitivity by using disks].

A review of methods for determination of sensitivity of nonsporulating anaerobic bacteria to antibacterial drugs with paper disks is presented and the prospects for using the method of cultivation in solid media in Petri dishes under a polymer film are discussed. It is shown that cultivation at a temperature of 42 degrees C for obtaining earlier results instead of the temperature of 37 degrees C usually used for cultivation has no advantages. With an account of the result variability depending on the inoculation dose and other factors it is necessary to determine the boundary values of the growth inhibition zones for resistant, moderate sensitive and sensitive strains with respect to every antibiotic.

[Determination of the ecological niche of Bacteroides in the gastrointestinal tract--a study of a model of sterile intestines in germ-free animals].

The interrelations of the population of microorganisms belonging to the genus Bacteroides with the structures of the mucous membrane of the gastrointestinal tract of germ-free guinea pigs and mini-pigs have been studied. A considerable part of the population of these anaerobic microorganisms is associated in some way with the intestinal mucosa; at least, quite a number of these organisms inhabit mucins covering the mucosa. The determination of this ecological niche occupied by bacteroids in the intestinal microbiocenosis suggests that these organisms have considerable influence on the physiological functions and pathological states of the digestive tract.

[Species composition of nonpigment-forming bacteroids isolated from the intestines of healthy persons and from clinical material].

The comparative study of the specific composition of bacteroids isolated from the intestine of healthy persons and from clinical material obtained from patients with different purulent inflammatory diseases has been carried out. This study has revealed that in the former case the prevalent bacteroid species are B. vulgatus and B.