Publications by authors named "T Ootaki"

Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate.

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Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that occurs commonly in old people. Hot spring radon therapy is widely practiced in Central Europe and Japan for relief from the painful symptoms. The usual duration of a spa treatment is a week or two, and the relief is temporary.

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We performed a study on renal cell carcinoma (RCC) detected by abdominal ultrasonography during routine health check-up at Sapporo Teishin Hospital. The study included a total of 13,127 ultrasonographic examinations performed on 4,339 patients who visited our hospital for routine health check-ups during a 21- year period from April 1995 to March 2016. RCC was suspected from ultrasonographic findings and later histopathologically diagnosed in 11 patients in this group (detection rate 0.

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Two different newly designed primer sets were used in PCR to amplify the fragments of chitin synthase (CHS) encoding genes (CHS) from the zygomycete fungus Phycomyces blakesleeanus, in which the cell wall is mainly made of chitin and chitosan, a deacetylated derivative from chitin. DNA sequencing and alignment analysis of the deduced amino acid sequences showed the possible existence of ten different genes. Six different DNA fragments, designated PbCHS1, PbCHS2, PbCHS3, PbCHS4, PbCHS5 and PbCHS6, homologous to chitin synthase genes were identified in 250-bp products.

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The sporangiophores of the zygomycete fungus Phycomyces blakesleeanus contain octahedral crystals with diameters of up to 5 micrometers in their vacuole. The crystals are associated with the intracellular membrane system. In tilted or horizontally placed sporangiophores, the crystals sediment to the respective lower face of the vacuole with a velocity of up to 100 micrometers per minute.

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