Publications by authors named "T Ntallaris"
Article Synopsis
- Extraction of epididymal sperm from bulls, particularly from rare or endangered breeds, is essential but often results in low-quality samples.
- Two methods were tested for extracting spermatozoa: a small incision in the epididymis and flushing the epididymis.
- Both methods yielded similar sperm quality metrics before and after thawing, indicating that either method is effective for gathering bull epididymal spermatozoa.
The objective of this study was to assess the ability of camel spermatozoa to bind in the Hyaluronan Binding Assay (HBA), to determine if conventional sperm quality parameters, in vitro fertilization capacity, and precursor of A-Kinase Anchoring Protein 4 (proAKAP4) values correlate with HBA results. The potential to predict post-thaw fertilization performance from HBA for fresh dromedary camel sperm was also evaluated. Semen samples were collected and assessed both fresh and post thawing, at 0 h and 1.
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- The study investigates the relationship between various in vitro sperm characteristics and stallion fertility, aiming to establish reliable fertility biomarkers for equine breeding.* -
- Key findings show positive correlations between pregnancy rates and sperm straightness, as well as living hydrogen peroxide positive sperm, while negative correlations involve lateral head displacement and dead superoxide positive sperm.* -
- The research suggests that factors like sperm motility and membrane integrity could serve as fertility indicators, but more data is needed to develop a reliable fertility prediction model.*
Addition of antibiotics to semen extenders was taken for granted for many years, from the time that commercial artificial insemination in livestock first began many decades ago. However, there is now a growing realisation that this non-therapeutic utilisation of antibacterial agents is contrary to current recommendations for prudent use that medical and veterinary professionals are advised to follow. Furthermore, antibiotics are not benign, having negative effects on sperm samples, the inseminated female, personnel and potentially the environment.
View Article and Find Full Text PDFAlthough previous studies have examined the relationship between the sperm DNA fragmentation index and fertility in stallions, other aspects of chromatin structure or packaging and fertility have not been explored. In the present study, relationships between fertility and DNA fragmentation index, protamine deficiency, total thiols, free thiols and disulfide bonds in stallion spermatozoa were investigated. Ejaculates (n = 36) were collected from 12 stallions and extended to prepare semen doses for insemination.
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