Violaceoid F induces nuclear translocation of FOXO3a by inhibiting CRM1 via a novel mechanism and suppresses HeLa cell growth.

FOXO3a is a transcription factor involved in cell growth inhibition and apoptosis. FOXO3a is localized in the cytoplasm in cancer cells, and its nuclear translocation by small molecules is expected to prevent cancer cell growth. In this study, we screened a fungal broth library in HeLa cells using fluorescently labeled FOXO3a and an AI-based imaging system.

Mechanical Effect of an Implant Under Denture Base in Implant-Supported Distal Free-End Removable Partial Dentures.

: In recent years, implant-assisted removable partial dentures (IARPDs) have been used clinically. However, the extent to which additional implants reduce the burden of supporting tissues is unclear. The aim of this study was therefore to investigate the influence of implanted IARPDs on stress sharing among supporting tissues, using finite element (FE) analysis.

Total synthesis, stereochemical assignment, and biological evaluation of opantimycin A and analogues thereof.

Opantimycin A, a rare antimycin-class antibiotic without the macrolide core, was isolated from sp. RK88-1355 in 2017. In this study, we explored the total synthesis and stereochemical assignment of opantimycin A.

Iron-sulphur protein catalysed [4+2] cycloadditions in natural product biosynthesis.

To the best of our knowledge, enzymes that catalyse intramolecular Diels-Alder ([4+2] cycloaddition) reactions are frequently reported in natural product biosynthesis; however, no native enzymes utilising Lewis acid catalysis have been reported. Verticilactam is a representative member of polycyclic macrolactams, presumably produced by spontaneous cycloaddition. We report that the intramolecular [4+2] cycloadditions can be significantly accelerated by ferredoxins (Fds), a class of small iron-sulphur (Fe-S) proteins.

Production of Kinanthraquinone D with Antimalarial Activity by Heterologous Gene Expression and Biotransformation in TK23.

Two new compounds, kinanthraquinone C () and kinanthraquinone D (), were isolated along with two known compounds, kinanthraquinone () and kinanthraquinone B (), produced by the heterologous expression of the biosynthetic gene cluster and its pathway-specific regulator, , in TK23. The chemical structures of compounds and were determined using mass spectrometry and nuclear magnetic resonance analyses. To examine a biosynthetic pathway of compounds and , incubation experiments were conducted using TK23 to supply the compounds and .