The metabolic fingerprint of Scots pine-root and needle metabolites show different patterns in dying trees.

The loss of leaves and needles in tree crowns and tree mortality are increasing worldwide, mostly as a result of more frequent and severe drought stress. Scots pine (Pinus sylvestris L.) is a tree species that is strongly affected by these developments in many regions of Europe and Asia.

Recombinant Oxidase from as a Potential Tool for Aflatoxin B1 Degradation in Contaminated Cereal Grain.

Forage grain contamination with aflatoxin B1 (AFB1) is a global problem, so its detoxification with the aim of providing feed safety and cost-efficiency is still a relevant issue. AFB1 degradation by microbial enzymes is considered to be a promising detoxification approach. In this study, we modified an previously developed GS115 expression system using a chimeric signal peptide to obtain a new recombinant producer of extracellular AFB1 oxidase (AFO) from (the yield of 0.

Constitutive inorganic pyrophosphatase as a reciprocal regulator of three inducible enzymes in Escherichia coli.

Background: Previous studies have demonstrated the formation of stable complexes between inorganic pyrophosphatase (PPase) and three other Escherichia coli enzymes - cupin-type phosphoglucose isomerase (cPGI), class I fructose-1,6-bisphosphate aldolase (FbaB) and l-glutamate decarboxylase (GadA).

Methods: Here, we determined by activity measurements how complex formation between these enzymes affects their activities and oligomeric structure.

Results: cPGI activity was modulated by all partner proteins, but none was reciprocally affected by cPGI.

A novel, cupin-type phosphoglucose isomerase in Escherichia coli.

Background: Escherichia coli cells contain a homolog of presumed 5-keto-4-deoxyuronate isomerase (KduI) from pectin-degrading soil bacteria, but the catalytic activity of the E. coli protein (o-KduI) was never demonstrated.

Methods: The known three-dimensional structure of E.

An Extract Purified from the Mycelium of a Tomato Wilt-Controlling Strain of Can Protect Wheat against Fusarium and Common Root Rots.

An approach to manage seed-transmitted Fusarium crown-foot-root rot (FCR, spp.) and common root rot (CRR, ) on wheat, avoiding environmental risks of chemicals, is seed treatments with microbial metabolites. strain FS-94 that induces resistance to tomato wilt was shown by this study to be a source of non-fungitoxic wheat-protecting metabolites, which were contained in a mycelium extract purified by gel-chromatography and ultrafiltration.