Publications by authors named "T Nashida"
Many elderly patients have oral dryness; thus, it is necessary to evaluate the oral moisture in a clinical setting. The aim of this study was to clarify the importance of controlling the measuring pressure of the oral moisture-checking device. The influence of the measuring pressure of the oral moisture-checking device was examined using agar under 10 measuring pressure conditions (Kruskal-Wallis test).
View Article and Find Full Text PDFEarly detection of oral disease is important to reduce its severity and increase the likelihood of successful treatment. This study aimed to perform a quantitative assessment of the saliva components as a first stage of the research to screen oral homeostasis. Here, saliva secretions collected from children were evaluated, and their constituents were analyzed to investigate the potential correlations between the buffering capacity and a range of salivary factors.
View Article and Find Full Text PDFThe Role of Genetic Factors in the Outbreak Mechanism of Dental Caries.
J Clin Pediatr Dent
March 2018
Objective: The aim of the present study was to investigate the relationships between cariogenic bacterial infection and single nucleotide polymorphisms (SNPs) in candidate genes associated with dental caries, and to explore the factors related to caries in children.
Study Design: Children aged 3 to 11 years were selected. Detection of cariogenic bacteria (Streptococcus mutans, Streptococcus oralis, Streptococcus sobrinus and Lactobacillus) from the plaque of each patient, and SNP analyses of five candidate genes (MBL2, TAS2R38, GLUT2, MMP13 and CA6) were performed using DNA isolated from buccal mucosal cells.
Article Synopsis
- A study confirmed the presence of the antibacterial protein BPIFB1 in the parotid glands of non-obese diabetic (NOD) mice, which model Sjögren's syndrome, revealing its protein expression levels for the first time.
- BPIFB1 was found to be localized in secretory granules of NOD mice's parotid glands, but was almost absent in control mice, indicating a significant difference in expression.
- Additionally, the protein was detectable in the saliva of NOD mice, suggesting its potential use as a diagnostic marker in further research.
Objective: To define the increased mRNA expression of Bpifb1, a member of the bactericidal/permeability-increasing protein family, in parotid acinar cells from non-obese diabetic (NOD) mice, an animal model for Sjögren's syndrome.
Materials And Methods: Parotid acinar cells were prepared from female NOD (NOD/ShiJcl) mice with or without diabetes, as well as from control (C57BL/6JJcl) mice. Total RNA and homogenate were prepared from the parotid acinar cells.