Screening and production of a potential extracellular fungal laccase from : Media optimization by response surface methodology (RSM) and central composite rotatable design (CCRD).

This paper describes the isolation of potent extracellular-laccase producing white-rot fungus, identified by 18 s-rRNA as Penicillium-chrysogenum and its medium optimization by central-composite-rotatable-design using RSM. The optimum laccase-activity of 6.0 U ml was obtained and maximum activity of 7.

Prevalence of coagulase negative Staphylococcus species among hospital personnel and surgical patients.

Two hundred and fourteen strains of Coagulase negative Staphylococci (CNS) isolated from hospital personnel (114 strains) and patients (100 strains) were identified upto species level. The prevalence rate of CNS in relation to sex, sources and type of specimens were analysed. Most of the CNS strains belonged to S.

Correlation between beta-lactamase production and MIC values against penicillin with coagulase negative staphylococci.

Two hundred strains of coagulase negative staphylococci (CNS) isolated from various clinical specimens (116) and healthy hospital personnel (84) were investigated for the production of beta-lactamases by means of three iodometric techniques and correlated with the minimum inhibitory concentration (MIC) values of penicillin-G by agar dilution technique and disc diffusion technique. One hundred and fifty (75.0%) of the 200 strains tested produced beta-lactamases.