Publications by authors named "T Nakazawa"
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-assisted genome editing has been applied to several major edible agaricomycetes, enabling efficient gene targeting. This method is promising for rapid and efficient breeding to isolate high-value cultivars and overcome cultivation challenges. However, the integration of foreign DNA fragments during this process raises concerns regarding genetically modified organisms (GMOs) and their regulatory restrictions.
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- - Hydrophobins are specialized proteins that help fungal mycelium manage its interaction with air and surrounding mediums, with specific genes identified in the fungus Pleurotus ostreatus, particularly those encoding Vmh2, Vmh3, and Hydph16, which play key roles in its growth.
- - The study found that deleting the gene for Hydph16 resulted in a significant reduction in aerial mycelium density and a 40% decrease in cell wall thickness compared to control strains, while leaving major cell wall polysaccharide components intact.
- - Unlike Vmh2 and Vmh3, the absence of Hydph16 did not affect mycelial hydrophobicity, indicating that different hydrophobins
In this study, we developed a mycelial dispersion strain by disrupting the pkac2 gene in the white-rot fungus Pleurotus ostreatus. pkac2 is a catalytic subunit gene of protein kinase A, which regulates several transcription factors related to cell wall synthesis. Liquid cultures of the Δpkac2 strains showed very high mycelial dispersibility and were visibly different from the wild-type (WT) strain.
View Article and Find Full Text PDFPurpose: The purpose of this study was to investigate biologically meaningful endotypes of open-angle glaucoma (OAG) by applying unsupervised machine learning to plasma metabolites.
Methods: This retrospective longitudinal cohort study enrolled consecutive patients aged ≥20 years with OAG at Tohoku University Hospital from January 2017 to January 2020. OAG was confirmed based on comprehensive ophthalmic examinations.
The regulatory mechanisms governing expression of genes encoding lignin-modifying enzymes (LME) in white-rot fungi remain largely unexplored. Although molecular cloning has identified CCAAT-boxes frequently located 5'-upstream of these genes, their role in transcriptional regulation is not well understood. This study examines the function of hap2, a gene encoding a hypothetical protein homologous to a component of the CCAAT-binding Hap complex, in the white-rot fungus Pleurotus ostreatus.
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