A multivariate analysis of prognostic indicators in complete hydatidiform moles (CHM).

Objective: To analyse prognostic factors in complete hydatidiform moles using multiple logistic regression analysis.

Methods: Evaluation of host and tumour related parameters including (a) gestational age, patient age, parity, molar phenotype, grade of proliferation of the tumour and cytological atypia, (b) expression of beta-HCG, EGF, EGFR, TGF-alpha, TGF-beta, IL1-alpha, IL1-beta by immunohistochemistry, (c) serial monitoring of serum beta-HCG levels by ELISA, and (d) lectin binding using jack fruit lectin histochemistry as indices for persisting trophoblastic disease (PTD).

Results: Serum beta-HCG levels at 4 weeks, cellular atypia, lectin binding, expression of TGF-alpha and IL1-beta showed highly significant correlation with persistence of the tumour (P<0.

Immunolocalization and quantitation of transforming growth factor alpha in hydatidiform mole.

Complete hydatidiform mole (CHM), a condition related to abnormal gestation, occurs predominantly in the young reproductive age group and has a high prevalence rate in the Trivandrum region, occurring in 1.2% of deliveries. Transforming growth factor alpha (TGF-alpha) is an important growth regulatory molecule, the location and function of which at the human fetomaternal interface in CHM remains to be determined.

Proliferating cell nuclear antigen (PCNA) expression in gestational trophoblastic diseases (GTD).

Gestational Trophoblastic Disease is an abnormal condition of the placenta, the incidence of which is very high in the state of Kerala, India. The proliferative rate of molar placentas in comparison with the normal placentas of comparable gestational age group was done in order to find out its role in the prognosis of this tumor by assessing the expression of PCNA in trophoblasts. PCNA expression was evaluated in 149 trophoblastic tumors and 96 normal placental tissue.

ras and c-myc oncoproteins during tumor progression in the uterine cervix.

Aims And Background: Altered oncogenic activity is a feature associated with many malignant and premalignant conditions. Among the many oncogenes, ras and myc are commonly altered in many tumors. This study aims to evaluate the expression of ras and c-myc oncoproteins in a total of 204 cervical tissue samples, including premalignant and malignant lesions as well as apparently normal cervical tissue.

Lack of association of gestational trophoblastic diseases (GTD) with syphilis and AIDS.

The association between human immunodeficiency virus (HIV) infection and syphilis infection as an etiological factor in Gestational Trophoblastic Disease (GTD) was investigated by means of micro-enzyme linked immunosorbent assay (Micro-ELISA) and Treposcreen-Rapid Plasma Reagin Card Test in 138 sera from patients with Gestational Trophoblastic Disease. We have found only one sample to be positive for HIV infection and one for VDRL. These findings suggest a lack of an etiologic role for the HIV and Syphilis infection in GTD.