[Effect of pyruvate, threonine, and phosphoethanolamine on acetaldehyde metabolism in rats with toxic liver injury].

Pyruvate dehydrogenase, threonine aldolase and phosphoethanolamine lyase can produce acetaldehyde during normal metabolism. We studied the effect of loading with the substrates of these enzymes (pyruvate, 500 mg/kg, i.p.

Influence of pyruvate, threonine and phosphoethanolamine on activities of some acetaldehyde-producing enzymes.

Threonine (50 mg/100 g, i.p.) leads to increased hepatic threonine aldolase activity in rats, although endogenous ethanol concentrations remain stable.

[Effect of pyrazole on the activity of acetaldehyde-producing enzymes in the liver].

Influence of pyrazole on the endogenous ethanol level and activities of acetaldehyde-producing enzymes was investigated. Drastic enhancement of the endogenous ethanol level in the blood and tissues was accompanied by an insignificant increase of phosphoethanolamine lyase activity, while activity of threonine aldolase and pyruvate dehydrogenase was unchanged.

[Effects of nicotinamide and oxythiamine on the lipogenic parameters of the adipose tissue of mice with non-insulin-dependent type of experimental diabetes mellitus and hyperinsulinemia].

The influence of nicotinamide and oxythiamine on the activity of NADPH-producing dehydrogenases of glucoso-6-phosphate, 6-phosphogluconate, malate, isocitrate, as well as concentration and synthesis rate of fatty acids in fatty tissue were studied in experiments on mice with genetically conditioned non-insulin-dependent diabetes and hyperinsulinemia. It has been established that in these diseases the synthesis of fatty acids and their inclusion into lipids are activated without increasing the above enzymes activity. It is shown that nicotinamide and oxythiamine inhibit inclusion of C from glucose into free fatty acids, antivitamin intensifies lipolysis in the fatty tissue of the diseased animals.

[The role of niacin in regulating the pentosophosphate pathway and production of NADP-H in fatty tissue].

A study was made of the effect of alimentary deficiency of niacin and of exogenous nicotinamide (500 mg/kg) on the activity of the key enzymes of the pentose phosphate pathway and NADP-dependent malate and isocitric dehydrogenase in the epididymal fatty tissue of rats. It is established that vitamin depletion in the animals' body brings about a 3-fold decrease in the content of NADP+ and a 1.7-fold decrease in the content of NADPH, a 43-percent inhibition of the activity of glucose 6-phosphate dehydrogenase and a 39-percent reduction with respect to transketolase.