To Stay or to Leave: The Role of School, Family, and Prosocial Goals in Migration Intentions of Russian High School Students.

Background: Migration intentions are extremely common among modern youth practically all around the globe. They do not always result in actual migration, but they should definitely be considered as an important indicator for the society, since the potential loss of the most valuable human resources entails long-term consequences for the development of a country or region. This study aims to examine the system of psychological factors that determine the desire of young people to stay in their region.

CRISPR/Cas9-Mediated Genome Editing of the Komagataella phaffii to Obtain a Phytase-Producer Markerless Strain.

Using CRISPR/Cas9 system, the recipient strains K. phaffii VKPM Y-5013 (His phenotype) and K. phaffii VKPM Y-5014 (Leu phenotype) were derived from the K.

Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed in Pichia pastoris.

Phytases are valuable industrial enzymes widely used in animal feed production, and when expressed in yeast, they undergo glycosylation. Herein, heterologous expression in Pichia pastoris and biochemical characterisation of glycosylated and deglycosylated forms of a novel phytase from Cronobacter turicensis belonging to the histidine acid phosphatase family were successfully carried out. Mutants with deleted N-glycosylation sites (N136, N171, and N202) were constructed by site-directed mutagenesis and characterised.

Optimistic Attributional Style as a Predictor of Well-Being: Exploring the Mediating Roles of Gratitude and Savoring the Moment.

Background: The construct of attributional style refers to the specific ways people explain events, both positive and negative. An optimistic attributional style (OAS) for negative events has been shown to be reliably associated with low rates of depression (Peterson et al., 1985; Sweeney et al.

A novel phytase from Citrobactergillenii: characterization and expression in Pichia pastoris (Komagataella pastoris).

The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (73.