The Nature and Clinical Significance of Atypical Mononuclear Cells in Infectious Mononucleosis Caused by the Epstein-Barr Virus in Children.

Atypical mononuclear cells (AM) appear in significant numbers in peripheral blood of patients with Epstein-Barr virus (EBV)-associated infectious mononucleosis (IM). We investigated the number and lineage-specific clusters of differentiation (CD) expression of atypical mononuclear cells in 110 children with IM using the anti-CD antibody microarray for panning leukocytes by their surface markers prior to morphology examination. The AM population consisted primarily of CD8+ T cells with a small fraction (0%-2% of all lymphocytes) of CD19+ B lymphocytes.

[Advantages of dose calculation by the Monte Carlo method for the treatment of patients with lung, and head and neck tumors with the robotic system CyberKnife ].

This paper analyzes the recalculation of plans for the exposure of patients with tumors of the lung, head and neck by the Monte Carlo method. There are presented the results of calculations with understating the dose by 29% when using the algorithm Ray-Tracing. It is proposed mandatory recalculation of dose by Monte Carlo method in planning exposure for patients with tumors of the lung and head and neck tumors to eliminate significant systematic errors in the values of input dose.

Rat eggs cannot wait: Spontaneous exit from meiotic metaphase-II arrest.

Mammalian eggs await fertilisation while arrested at the second metaphase stage of meiotic division. A network of signalling pathways enables the establishment and maintenance of this metaphase-II arrest. In the absence of fertilisation, mammalian eggs can spontaneously exit metaphase II when parthenogenetically stimulated, or sometimes without any obvious stimulation.

Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development.

Methylation of cytosine is a DNA modification associated with gene repression. Recently, a novel cytosine modification, 5-hydroxymethylcytosine (5-hmC) has been discovered. Here we examine 5-hmC distribution during mammalian development and in cellular systems, and show that the developmental dynamics of 5-hmC are different from those of 5-methylcytosine (5-mC); in particular 5-hmC is enriched in embryonic contexts compared to adult tissues.

[Manifestations of colonic dysbacteriosis in patients with drug-resistant respiratory tuberculosis].

The bacteriological and clinical manifestations of colonic dysbacteriosis were studied in 65 patients with drug-resistant respiratory tuberculosis before treatment with reserve drugs. Colonic microbiological disturbances corresponding to first-, second-, and third-degree dysbacteriosis were detected in 6 (9%), 14 (21%), and 42 (65%), respectively; no evidence of dysbacteriosis was found in 3 (5%) patients. There were characteristic clinical symptoms of dysbacteriosis (tympanism in 80% of the patients, signs of dyspepsia in 73.