Effect upon mortality of the extension to holidays and weekends of the "ICU without walls" project. A before-after study.

Objective: To determine whether extension to holidays and weekends of the protocol for the early proactive detection of severity in hospital ("ICU without walls" project) results in decreased mortality among patients admitted to the ICU during those days.

Design: A quasi-experimental before-after study was carried out.

Setting: A level 2 hospital with 210 beds and a polyvalent ICU with 8 beds.

Effect of the timing of admission upon patient prognosis in the Intensive Care Unit: On-hours versus off-hours.

Objective: To assess the repercussion of the timing of admission to the ICU upon patient prognosis.

Design: A prospective, observational, non-interventional cohort study was carried out.

Scope: A second level hospital with 210 operational beds and a general ICU with 8 operational beds.

A complete BAC-based physical map of the Arabidopsis thaliana genome.

Arabidopsis thaliana is a small flowering plant that serves as the major model system in plant molecular genetics. The efforts of many scientists have produced genetic maps that provide extensive coverage of the genome (http://genome-www. stanford.

Use of the IGF BAC library for physical mapping of the Arabidopsis thaliana genome.

In order to generate a physical map of the Arabidopsis thaliana genome based on bacterial artificial chromosome clones (BACs), an iterative high throughput hybridisation strategy was applied and its efficiency was evaluated. Thus, probes generated from both ends of 500 BAC clones selected from the Arabidopsis-IGF-BAC library were hybridised to the entire library gridded on high density filters. The 1000 hybridisation reactions identified 4496 clones (41.

Construction and characterization of the IGF Arabidopsis BAC library.

A bacterial artificial chromosome (BAC) library has been established for Arabidopsis thaliana (ecotype Col-0) covering about seven haploid nuclear genome equivalents. This library, called the Institut für Genbiologische Forschung (IGF) BAC library, consists of 10,752 recombinant clones carrying inserts (generated by partial EcoRI digestion) of an average size of about 100 kb in a modified BAC vector, pBeloBAC-Kan. Hybridization with organellar DNA and nuclear repetitive DNA elements revealed the presence of 1.