[Demonstration and classification of heterogeneity of phosphoprotein phosphohydrolase in swine spleen extracts based on sodium fluoride inhibition].

The heterogeneity of phosphoprotein phosphohydrolase--Fpf in cellular free spleen extracts of pigs may be determined by sodium fluoride inhibition. NaF concentration of 5 X 10(-3) mol/l divides Fpf into 3 groups and shows a wide inhibition range from 15 to 85% of the initial activity of the enzyme. Naf concentration of 5 X 10(-4)mol/l stops Fpf activity in the range from 10 to 65% and divides it into two groups.

Occurrence and characteristics of sclerotic lesions in uterine arteries of sterile and multiparous pigs.

Sows and gilts of the Great White Polish (GWP) breed were classified into groups of 10 each: gilts 8 mo of age (A), sows over 1 yr old with an average litter size of five (BI) and eleven (BII), 2- to 3-yr-old sows with an average litter size of five (CI) and nine (CII), 4- to 6-yr-old sows with an average litter size of six (DI) and nine (DII). Gilts of group A were necropsied on d 10 of the estrous cycle while sows were necropsied 120 to 360 d postpartum. A blood sample obtained by vena cava puncture immediately before exsanguination was quantitated for total cholesterol, free cholesterol, cholesterol ester, total lipid, triglyceride, high and low density lipoprotein and chylomicrons.

[Proteolytic activity of blood plasma and hemolysed blood in dogs after acute chlorophos poisoning].

On dogs under Eunarkon anesthesia (0,03 ml Eunarkon/kg body weight) after one intramuscular lethal injection of phoschlor (500 mg/lg body weight = LD100) the proteolytic activity (APR) was estimated in the blood plasma at pH 3,5-APR-3,5 and in the hemolyzed blood at pH-7,4-APR-7,4. The results showed that heavy poisoning with phoschlor led to metabolic disturbances in the proteolytic system of the blood plasma and blood, the expression of which was increasing in the course of time a statistically important increase (alpha = 0,001) of APR-3,5 and APR-7,4. APR induction in blood is not a result of direct activation of enzymes by pesticides.

[Influence of coumarin on the lipolytic activity in the hyperlipaemic plasma of cocks].

Two groups of cocks were kept for 84 days on a diet rich in fat (group D) or on a similar diet with addition of coumarin (group DK). In the plasma of these birds there occurred a distinct increase of the concentration of total fat (TC) and cholesterol (CH), in comparison with the concentration of these substances of the plasma of control birds. The concentration of TC in the plasma of group DK was higher than that observed in group D.