The role of chloroplast and cytoplasm in the NADP-glutamate dehydrogenase and glutamine synthetase synthesis in Ankistrodesmus cells.

The effects of transcription and translation inhibitors on NADP-glutamate dehydrogenase and glutamine synthetase synthesis in nitrogen-starving Ankistrodesmus braunii cells have been studied. Considering the results obtained one can suggest that both enzymes are coded in the chloroplast genome and that during nitrogen starvation specific mRNA's are partly transferred from the chloroplast into the cytoplasm and can be translated there on 80S ribosomes.

The role of chloroplast and cytoplasm in the NADP-glutamate dehydrogenase and glutamine synthetase synthesis in Ankistrodesmus cells.

The effects of transcription and translation inhibitors on NADP-glutamate dehydrogenase and glutamine synthetase synthesis in nitrogen-starving Ankistrodesmus braunii cells have been studied. Considering the results obtained one can suggest that both enzymes are coded in a chloroplast genome and that during nitrogen starvation specific mRNA's are partly transferred from chloroplast into cytoplasm and can be translated there on 80S ribosomes.

[Ferredoxin-dependent glutamate synthase of Chlorella].

Ferredoxin-dependent glutamate synthase has been found in cells of thermophylic Chlorella strain Ch. pyrenoidosa 82T. The enzyme is active with its own ferredoxin and that of Spirulina.

[Glutamate dehydrogenases of unicellular green algae: effects of nitrate and ammonium in vivo].

The constitution and control by the inorganic nitrogen source of glutamate dehydrogenases of some unicellular green algae have been studied. The Ankistrodesmus braunii and Scenedesmus obliquus cells contain two different glutamate dehydrogenases, one of which is NADP-specific, the other is active with both NAD and NADP. Their synthesis does not depend on the nitrogen source.