The aim of the study was to develop a sensitive and specific method for revealing the direct marker of hepatitis C virus (HCV)--core protein in the serum and to test it in the laboratory setting. Experiments were made on plasma and serum samples from asymptomatic HCV-seropositive blood donors (n=65), patients with acute (AHC) and chronic (CHC) hepatitis C (n=295), and HCV-seronegative blood donors (n=20). The processing protocol for serum included their concentration by means of polyethylene glycol and subsequent treatments of pellets to detect core protein in free virions, nonenveloped nucleocapsids, and immune complexes.
View Article and Find Full Text PDFDuring a natural hygienic experiment, the physical development, psychomotor activity, exercise performance, and readiness were evaluated in 6-year-old children, in whom physical education had been organized by routine and experimental programs, including health-improving swimming in the indoor pool of a preschool educational establishment. Exercises built up on the principle of plot-role playing games, by using the developing corrective exercises and psychological and pedagogical escort, were established to be highly effective. The proposed methods contribute to the timely harmonious development of a child, his movement characteristics, positively affect the neurofunctional status, by ensuring the optimum psychomotor development, and maintain a high exercise performance.
View Article and Find Full Text PDFThe accumulation of individual hepatitis C virus (HCV) proteins in the liver cells of patients with acute hepatitis C (AHC) and their association with the course and outcome of the disease were studied. AHC protein expression in the cryostat liver sections from 20 patients with AHC was estimated by immunohistochemical assay using original monoclonal antibodies to 5 HCV proteins (core, NS3, NS4A, NS4B, and NS5A). The results of HCV detection in the patients were compared with their biochemical, clinical, and morphological findings.
View Article and Find Full Text PDFIntracellular recordings have shown that there are three types of receptors in the retina of the locust with lambda max = 360, 430 and 530 nm. Their spectral sensitivity curves are considerably wider than the absorption curves of the corresponding pigments. On the basis of spectral sensitivity curves obtained and assuming that each receptor contains only one pigment, possible coefficients of electrical coupling between receptors with different spectral characteristics are calculated.
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