Publications by authors named "T M Theilen"
Ataxia telangiectasia (AT) is a rare autosomal-recessive disorder characterized by profound neurodegeneration, combined immunodeficiency, and an increased risk for malignant diseases. Treatment options for AT are limited, and the long-term survival prognosis for patients remains grim, primarily due to the emergence of chronic respiratory pathologies, malignancies, and neurological complications. Understanding the dysregulation of the immune system in AT is fundamental for the development of novel treatment strategies.
View Article and Find Full Text PDFDiagnosis of Hirschsprung disease by analyzing acetylcholinesterase staining using artificial intelligence.
J Pediatr Gastroenterol Nutr
September 2024
Article Synopsis
- Classical Hirschsprung disease (HD) is characterized by the lack of nerve cells in the colon, diagnosed via rectal biopsy showing aganglionosis and cholinergic hyperinnervation, but biopsy methods can affect accuracy.
- A study analyzed 190 samples from patients using digital imaging software to assess acetylcholinesterase (AChE) staining and utilized machine learning to detect patterns of hyperinnervation.
- Results indicated that AChE staining was significantly higher in HD patients than in healthy individuals, and machine learning models demonstrated high accuracy in diagnosing HD, particularly when excluding non-rectal samples.
Fluorescence confocal microscopy (FCM) is an optical technique that uses laser light sources of different wavelengths to generate real-time images of fresh, unfixed tissue specimens. FCM allows histological evaluation of fresh tissue samples without the associated cryo artifacts after frozen sectioning. The aim of this study was to prospectively evaluate pediatric tumor specimens and assess their suitability for fresh tumor sampling.
View Article and Find Full Text PDFPurpose: Abdominal wall closure in patients with giant omphalocele (GOC) and complicated gastroschisis (GS) remains to be a surgical challenge. To facilitate an early complete abdominal wall closure, we investigated the combination of a staged closure technique with continuous traction to the abdominal wall using a newly designed vertical traction device for newborns.
Methods: Four tertiary pediatric surgery departments participated in the study between 04/2022 and 11/2023.