[Motifs in the primary structure of MHC Class I molecules and their use for the design of synthetic T-cell receptor ligands].

This paper attempts to describe a great variety of allelic forms of mammalian major histocompatibility gene complex (MHC) Class I molecules as a single "formula" that contains information on motifs in their primary structure. The positions of amino acid residues that are invariant even in highly evolutionarily different species have been found in the structure of MHC Class I molecular domains that are recognizable by T-cell receptors (TCR). The spatial arrangement of invariant residues in the tertiary structure of MHC molecules is indicative of their structure-forming function: they are generally located at the sites of contact of alpha-helices and beta-sheets.

Cross reactivity of T cell receptor on memory CD8+ cells isolated after immunization with allogeneic tumor cells.

Experiments on mice deficient in expression of class I major histocompatibility complex molecules showed that memory CD8+ cells recognizing the alloantigen by the direct allogeneic recognition mechanism selectively proliferated in response to heated allogeneic cells. Adoptive transfer of memory cells from mice expressing green fluorescent protein transgene to wild-type animals showed for the first time that long-living memory cells suppress the response of naive T cells and abolish their involvement in the pool of memory cells. The pool of long-living memory T cells was obtained in vitro with heated allogeneic stimulators.

[Photoinactivation of microorganisms in the presence of photosensitizers].

Two photosensitizing preparation for the photodynamic therapy of oncological diseases have been obtained. As revealed in this study, under experimental conditions their action suppresses the growth of Enterococcus faecalis and Staphylococcus aureus, which may be of great importance for the treatment of infections. And in contrast to antibiotic therapy, their use may not affect the development of normal intestinal microflora.

[New type II and IIs restriction endonucleases from thermophilic bacteria of the genus Bacillus].

Restriction endonucleases have been isolated from 26 strains of thermophilic strains of the Bacillus genus, their recognition sequences were determined, and for 15 of them cleavage sites identified. The enzymes proved to be isoschizomers of known endonucleases BstNI, EarI, HaeIII, HpaII, Cfr10I, BsiYI, BclI, BbvII, BbvI, BstEII, BsaBI, BsrI, FspI, ClaI, SfeI.