Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials.

We investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon grown on mineral pyrite (FeS). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells and mineral materials. Therefore, we applied an improved protocol to break the microbial cells and separate their organic constituents from the mineral surface, to extract lipophilic compounds through liquid-liquid extraction, and performed metabolomics analyses using MALDI-TOF MS and UHPLC-UHR-Q/TOF.

Secondary vs. primary pituitary xanthogranulomas: which yellow is more mellow?

Pituitary xanthogranulomatomas (XG) are a rare pathological entity caused by accumulation of lipid laden macrophages and reactive granuloma formation usually triggered by cystic fluid leakage or hemorrhage. Our aim was to compare clinical characteristics and presenting features of patients with secondary etiology of XG and those with no identifiable founding lesion (primary -"pure" XG) in order to gain new insights into this rare pituitary pathology. In a retrospective review of 714 patients operated for sellar masses, at tertiary center, we identified 16 (2.

The causal influence of conscious engagement on photonic behavior: A review of the mind-matter interaction.

The well-known, quantum physics "double-slit" experiment was the first demonstration of wave-particle duality of light-photons naturally behave like waves, but once they are registered by a conscious observer they switch to behaving like particles. In recent years, a new avenue of research has reported a psychophysical interaction occurring when focused attention was employed in the double-slit experiment. In this context, the act of focusing attention to photons passing through the double-slit appears to collapse their wave function thus causing a shift toward particle-like behavior reflected in a decreased intensity of wave interference.

"Freezing" Thermophiles: From One Temperature Extreme to Another.

New detections of thermophiles in psychrobiotic (i.e., bearing cold-tolerant life forms) marine and terrestrial habitats including Arctic marine sediments, Antarctic accretion ice, permafrost, and elsewhere are continually being reported.

Thermoacidophilic Bioleaching of Industrial Metallic Steel Waste Product.

The continuous deposition of hazardous metalliferous wastes derived from industrial steelmaking processes will lead to space shortages while valuable raw metals are being depleted. Currently, these landfilled waste products pose a rich resource for microbial thermoacidophilic bioleaching processes. Six thermoacidophilic archaea (, and ) were cultivated on metal waste product derived from a steelmaking process to assess microbial proliferation and bioleaching potential.