Publications by authors named "T M Makris"
Article Synopsis
- * CADD utilizes its own amino acids (tyrosine and lysine) and operates with a unique Fe/Mn cofactor instead of the typical iron cofactor found in similar enzymes, impacting its mechanism of action.
- * Advanced studies reveal how CADD's structure and active-site variants affect its metal ion preferences, indicating that a transient tyrosine radical is involved in initiating pABA synthesis.
Article Synopsis
- The study aimed to assess the impact of the COVID-19 pandemic on hypertension awareness and treatment in Greece, analyzing data collected in 2019 and 2022 during the May Measurement Month (MMM) initiative.
- Data from over 12,000 adults revealed consistent hypertension prevalence rates (around 41-43%) before and after the pandemic, but an increase in undiagnosed cases was observed, rising from 21.3% to 27.5%.
- The findings suggest that while the overall prevalence of hypertension remained stable, there is a pressing need for improved national strategies to detect and manage hypertension effectively in Greece, especially post-pandemic.
A facile strategy is presented to enhance the accumulation of ferryl (iron(IV)-oxo) species in HO dependent cytochrome P450s (CYPs) of the CYP152 family. We report the characterization of a highly chemoselective CYP decarboxylase from Staphylococcus aureus (OleT) that is soluble at high concentrations. Examination of OleT Compound I (CpdI) accumulation with a variety of fatty acid substrates reveals a dependence on resting spin-state equilibrium.
View Article and Find Full Text PDFChlamydia protein associating with death domains (CADD), the founding member of a recently discovered class of nonheme dimetal enzymes termed hemeoxygenase-like dimetaloxidases (HDOs), plays an indispensable role in pathogen survival. CADD orchestrates the biosynthesis of -aminobenzoic acid (ABA) for integration into folate via the self-sacrificial excision of a protein-derived tyrosine (Tyr27) and several additional processing steps, the nature and timing of which have yet to be fully clarified. Nuclear magnetic resonance (NMR) and proteomics approaches reveal the source and probable timing of amine installation by a neighboring lysine (Lys152).
View Article and Find Full Text PDFThe cytochrome P450 (CYP) AspB is involved in the biosynthesis of the diketopiperazine (DKP) aspergilazine A. Tryptophan-linked dimeric DKP alkaloids are a large family of natural products that are found in numerous species and exhibit broad and often potent bioactivity. The proposed mechanisms for C-N bond formation by AspB, and similar C-C bond formations by related CYPs, have invoked the use of a ferryl-intermediate as an oxidant to promote substrate dimerization.
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