Publications by authors named "T M Khizhniakova"

Changes of the immune status are decisive in the pathogenesis of cytomegalovirus infection, which is a cause for the intrauterine infection. Therefore, complex immunological and virological examinations are advisable to make in pregnant women with compromised obstetrics history, pathological pregnancy course and with indirect signs of intrauterine fetus infection for the sake of choosing an optimal tactics of observation and treatment.

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Many in vitro and in vivo experiments using contemporary methods supported safety and efficiency of "VITA" device protecting humans and living beings from electromagnetic fields. Therefore, bioenergetic safety device "VITA" is advisable for use.

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Eighty eight autopsy specimens obtained from 30 fetuses, still-borns and infants died during the first year of life, all suspected for congenital virus infection at postmortem examination, were studied. The specimens were analyzed by 3 techniques: rapid culture method (RCM) for detection of cytomegalovirus (CMV) infectious activity, the immunocytochemical method for detection of CMV antigen in prints of organs and polymerase chain reaction (PCR) for detection CMV DNA. CMV was detected in 16 out of 26 specimens (61.

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A variant of Vero cell line has been obtained, adapted to the Russian Eagle's medium with 8% fetal calf serum. The variant is characterized by intensive cell proliferation, possesses a different content of chromosomes, contains no oncogens and contaminants, specifically, Mycoplasma. The strain was characterized and certified in accordance with the WHO requirements.

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Hepatitis A virus (HAV) was adapted to nonprimate BHK-21 cell line (Syrian hamster kidney). Enzyme immunoassay, immunoblotting, and slot hybridization demonstrated the capacity of HM175 culture strain to stable reproduction in this cell line. More than 50 passages of adapted HAV were carried out, which showed no changes in the basic cultural characteristics of the virus.

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