Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1.

With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.

Comminuted Paraspinal Rib Fractures with Resultant Impending Penetrating Aortic Injury Requiring Costovertebral Rib Fixation: A Case Report.

: Rib fractures are common in patients with trauma, and patients with multiple rib fractures often require surgical stabilization. Because rib fractures may occur at different sites along the ribs, the technical approach to surgical stabilization varies. Here, we present a case of posterior rib fractures with multiple paraspinal fragmented rib segments that were successfully treated with costovertebral plate fixation.

Reinterpretation of Conflicting ClinVar Missense Variants Using VarSome and CanVIG-UK Gene-Specific Guidance.

Background: The accurate interpretation of the /2 variant is critical for diagnosing and treating hereditary breast and ovarian cancers. ClinVar is a widely used public database for genetic variants. Conflicting classifications of pathogenicity can occur when different submitters categorize the same genetic variant inconsistently as pathogenic (PV), likely pathogenic (LPV), likely benign (LBV), benign (BV), or a variant of uncertain significance (VUS).

Burn Patient Education in the Modern Age: A Comparative Analysis of ChatGPT and Google Performance Answering Common Questions on Burn Injury and Management.

Patients often use Google for their medical questions. With the emergence of artificial intelligence large language models, such as ChatGPT, patients may turn to such technologies as an alternative source of medical information. This study investigates the safety, accuracy, and comprehensiveness of medical responses provided by ChatGPT in comparison to Google for common questions about burn injuries and their management.

Cryo-EM Structure of Human Hyaluronidase PH-20.

PH-20 is a specific type of hyaluronidase that plays a critical role in the fertilization process by facilitating the initial binding of sperm to the glycoprotein layer surrounding the oocyte and subsequently breaking down hyaluronic acid polymers in the cumulus cell layer. PH-20 contains an epidermal growth factor (EGF)-like domain, which may be involved in the recognition of the glycoprotein layer in addition to the catalytic domain. Herein, we report the structure of human PH-20 determined by cryogenic electron microscopy.