[Effect of changes in erythrocyte lipid spectrum on erythrocyte functional parameters in patients with diabetes mellitus].

Studies of the red cell lipid spectrum in diabetics and of the effects of bioactive substances and reconstruction of the red cell membrane lipid component on these cells aggregation have shown increased levels of lysophosphatidylcholine, cholesterol, cholesterol to phospholipid ratio, and saturated fatty acids in the presence of phosphatidylcholine reduction. Fluctuations in the levels of lipids of other classes were also revealed. Studies of red cell aggregation have revealed that incubation of donors' and diabetics' red cells with hormones did not lead to essential changes in the aggregation induced by alcian blue.

[The effect of changes in the lipid composition of membranes on the functional activity of platelets].

The phospholipid and fatty acid composition as well as the effect of platelet lipid composition modifications on the functional parameters of platelets were studied in blood sera from healthy donors and from patients with ischemic heart disease (IHD). It was found that the content of cholesterol and phospholipid hydrolysis products in IHD patients was increased. Reconstitution of the lipid composition of donor platelets by lysophosphatidylcholines, phosphatidic acid, fatty acids and cholesterol led to the increase of the platelet functional activity.

[Effect of lipids on thrombocyte aggregation in patients with ischemic heart disease].

Platelet lipid composition was studied in healthy subjects and patients with coronary heart disease (CHD) and effects of various phospholipids (PL) were examined on aggregatory parameters. The patients with CHD was found to show higher levels of lysophosphatidylcholine (LPC), phosphatidic acid (PA), cholesterol (Chol), increased Chol/PL of saturated fatty acids, elevated malonic dialdehyde and lower phosphatidylinositol (PI) concentrations. Addition of liposomes from various PL classes to platelet-rich plasma indicated as follows.

[Effect of changes in the membrane lipid composition on the activity of respiratory chain enzymes in rat liver mitochondria].

The lipid composition and fatty acid spectrum of individual phospholipid fractions of internal and external membranes of mitochondria was studied in alloxan diabetes. It was found that the phosphatidylserine content is reduced under these conditions, while those of lysophosphatidylcholines, diphosphatidylglycerols and cholesterol are increased, and the fatty acids are saturated with phospholipids. The observed changes in the lipid composition of membranes cause a decrease in the rate of oxygen consumption in various metabolic states as well as in the activity of NAD X H+-, succinate and cytochrome oxidases in rat liver mitochondria.

[Lipid content of mitochondrial membranes of the rat liver in alloxan diabetes].

Lipid content of liver mitochondrial membranes was studied in rats with alloxan diabetes. It was shown that the concentration of mitochondrial neutral lipids rose, whereas phospholipid content in the external membrane descended and that in the internal membrane ascended. The level of mitochondrial phospholipid saturated fatty acids tended toward increase.