Deletion mapping of the Aequorea victoria green fluorescent protein.

Aequorea victoria green fluorescent protein (GFP) is a promising fluorescent marker which is active in a diverse array of prokaryotic and eukaryotic organisms. A key feature underlying the versatility of GFP is its capacity to undergo heterocyclic chromophore formation by cyclization of a tripeptide present in its primary sequence and thereby acquiring fluorescent activity in a variety of intracellular environments. In order to define further the primary structure requirements for chromophore formation and fluorescence in GFP, a series of N- and C-terminal GFP deletion variant expression vectors were created using the polymerase chain reaction.

Medical-examiner-reported fatal occupational injuries, North Carolina, 1978-1984.

Fatal occupational injuries are a major public health problem in the U.S. Utilizing a medical examiner database from North Carolina, 1,233 fatal work-related injuries were identified in a 7-year time period.