PREVENTion and treatment of incontinence-associated dermatitis through a codesigned manual (PREVENT-IAD): a study protocol for a feasibility cluster randomised controlled trial with a nested process evaluation.

Introduction: Incontinence is commonly experienced by adults who receive care support in a residential facility or in their own home. These individuals are at risk of developing incontinence-associated dermatitis (IAD), which is caused by prolonged and repeated exposure of the skin to urine or faeces. An IAD manual was developed providing an evidence-based clinical algorithm and an e-learning training programme for the prevention and treatment of IAD.

Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells.

Phospholipid flippases in the P4-ATPase family are essential for establishing membrane asymmetry. These ATP-powered pumps translocate specific lipids from the exofacial leaflet to the cytosolic leaflet of the plasma membrane, thereby concentrating substrate lipids, such as phosphatidylserine, in the cytosolic leaflet while non-substrate lipids populate the exofacial leaflet. Here, we describe a method for measuring P4-ATPase transport activity in the yeast plasma membrane by using flow cytometry to quantify the uptake of lipids derivatized with a fluorescent [7-nitro-2-1,3-benzoxadiazol-4-yl)amino] (NBD) group on a short (C6) fatty acyl chain.

Stratified Medicine Paediatrics: Cell free DNA and serial tumour sequencing identifies subtype specific cancer evolution and epigenetic states.

We profiled a large heterogenous cohort of matched diagnostic-relapse tumour tissue and paired plasma-derived cell free DNA (cfDNA) from patients with relapsed and progressive solid tumours of childhood. Tissue and cfDNA sequencing results were concordant, with a wider spectrum of mutant alleles and higher degree of intra-tumour heterogeneity captured by the latter, if sufficient circulating tumour-derived DNA (ctDNA) was present. Serial tumour sequencing identified putative drivers of relapse, with alterations in epigenetic drivers being a common feature.

Genetics Adviser: The development and usability testing of a new patient digital health application to support clinical genomic testing.

Purpose: Increasing demand for genomic testing coupled with genetics workforce shortages has placed unsustainable pressure on standard models of care. Digital tools can offer improved access, efficiency, and cost savings. We created a patient-facing digital health application to support genomic testing.