Allelic polymorphism of cytokine genes during pulmonary tuberculosis.

Modern immunological and molecular genetic studies showed that tuberculosis is accompanied by an imbalance in the production of immunoregulatory cytokines by mononuclear leukocytes. T allele and homozygous TT genotype of T-330G polymorphism in the IL2 gene, T allele and TT genotype of C-590T polymorphism in the IL4 gene, and CC genotype of A-1188C polymorphism in the IL12B gene are immunogenetic factors that have protective activity against susceptibility to pulmonary tuberculosis. Susceptibility to tuberculous infection is associated with A1A2 genotype of the polymorphic region +3953 A1/A2 in the IL1B gene; G allele and TG and GG genotypes of T-330G polymorphism in the IL2 gene; C allele and CC and CT genotypes of C-590T polymorphism in the IL4 gene; and AC genotype of the polymorphic region A-1188C in the IL12 gene.

[Middle molecular weight peptides from rat blood serum during acute liver injury and administration of iodinated oil].

A spectrum of mean-molecular blood serum peptides in rats with experimental toxic hepatitis and parenteral injection of iodinated oil has been studied using the method of gel-filtration on a sephadex G-25. It is established that the toxic hepatitis is characterized by the expressed rearrangement of the stock of mean-molecular peptides with a preferential growth in the blood serum and normalization of their concentrations 24h after the injection of iodinated oil accompanied by attenuation of the organism intoxication phenomena. An increase in the content of mean-molecular peptides was registered 48 hours after the iodinated oil injection.

[Middle molecule fraction in the serum of rats with toxic hepatitis].

An increase in the amount of the middle molecular peptide (MM) fraction in the blood serum of rats with experimental toxic hepatitis and normalization of the MM pool after the administration of the amino acid mixture Alvesin "Novy" are shown. The chromatographic spectra of the MM fraction in normal and experimental animals are compared.

[Free amino acids in brain tissue and its subcellular structures in hyperthermia in rats].

Free amino acids reserves in the brain tissue, nuclei and mitochondria were investigated in mongrel albino rats under conditions of hyperthermia (45 degrees C) of different duration--7,20 and 60 min. It was found that reserves of most free amino acids in the albino rat brain decreased under a short-term (7 min) hyperthermia and accumulated under more prolonged (20-60 min) one. The amount of amino acids in the brain mitochondria increased 7 min after the experiment start and decreased 20 min later, then (60 min later) the amount of most amino acids increased considerably.